Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment

• Lester Uy Vinzons,
• Guo-Chung Dong and
• Shu-Ping Lin

Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96

• nanopillar substrate having the smallest CAs (CA ≈ 30°). Based on confocal fluorescence microscopy of immunostained samples (Figure 1J–L), laminin successfully adsorbed onto the O2 plasma-treated PU samples. There was good laminin coverage on all of the samples, even on the nanostructures, as indicated by

• replaced with fresh differentiation medium every two days. The neurite outgrowth experiments were performed in triplicate. The adsorbed laminin on the PU substrates was observed using confocal fluorescence microscopy. PC12 neurite outgrowth after differentiation was characterized using fluorescence

• values after plasma treatment, the lower CA [∥] values (<60°) indicate that the surface was hydrophilic enough along the groove direction, and good solution coverage could be achieved during laminin coating. We also confirmed laminin adsorption on the grooved PU samples using confocal fluorescence

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• to enhance the biological properties and improve cellular uptake of the MPs. The cellular uptake was also measured by fluorescence microscopy as shown in Supporting Information File 1, Figure S1. Conclusion Biodegradable albumin submicron particles were successfully loaded with CUR into protein

The origin of black and white coloration of the Asian tiger mosquito Aedes albopictus (Diptera: Culicidae)

• Manuela Rebora,
• Gianandrea Salerno,
• Silvana Piersanti,
• Alexander Kovalev and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2023, 14, 496–508, doi:10.3762/bjnano.14.41

• analysed using scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. Reflectance spectra of the white areas are measured. No clear difference is present in the morphology of micro- and nanostructures of black and white scales in SEM and TEM, but black scales contain a

• . The ultrastructure of the white and black scales on the hindlegs of Ae. albopictus is analysed using scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. Moreover, reflectance spectra of the white areas are measured. The scales are present also on other body

• ) Personal ref. no.: 91725732). Author Contributions The study was designed by all the authors. S.G. performed the cryo-scanning electron microscopy investigations. M.R. and S.P. performed the transmission electron microscopy investigations. G.S. performed the light microscopy and the fluorescence microscopy

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• ) [16]. Unfortunately, confocal imaging is limited by relatively low throughput (even with automation) and can be ambiguous when determining internalization within 500 nm of the cell membrane [17]. However, widefield fluorescence microscopy is still widely used when it comes to observing the expression

• sensing methods. Future Directions and Outlook Common techniques used to determine uptake and transfection possess limitations. Image-based techniques, such as widefield fluorescence microscopy and confocal microscopy, are limited by relatively low throughput and can be ambiguous for properly quantifying

• cells. (f) Annual prevalence of reporting imaging and (or) flow cytometry during the last five years. Of note, “imaging” refers to images captured by widefield fluorescence microscopy or confocal microscopy (electron microscopy excluded). See details about experimental approach and scope in Supporting

Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine

• Zoran M. Marković,
• Milica D. Budimir,
• Martin Danko,
• Dušan D. Milivojević,
• Pavel Kubat,
• Danica Z. Zmejkoski,
• Vladimir B. Pavlović,
• Marija M. Mojsin,
• Milena J. Stevanović and
• Biljana M. Todorović Marković

Beilstein J. Nanotechnol. 2023, 14, 165–174, doi:10.3762/bjnano.14.17

• assay by measuring the absorbance at 540 nm on Tecan Infinite 200 Pro multiplate reader (Tecan Group, Männedorf, Switzerland). The cytotoxicity results were presented as a percentage of the control (untreated cells), which was arbitrary set to 100%. Fluorescence microscopy Hela cells were grown in low

Antimicrobial and mechanical properties of functionalized textile by nanoarchitectured photoinduced Ag@polymer coating

• Jessica Plé,
• Marine Dabert,
• Helene Lecoq,
• Sophie Hellé,
• Lydie Ploux and
• Lavinia Balan

Beilstein J. Nanotechnol. 2023, 14, 95–109, doi:10.3762/bjnano.14.11

• (chosen for its inertness against microorganism proliferation and for the high image quality it provides with upright fluorescence microscopy). Liquid diffusion assay. The samples were placed in individual, sterile dishes and inoculated with 4 mL of E. coli or C. albicans suspensions of 0.1 OD600. Samples

Studies of probe tip materials by atomic force microscopy: a review

• Ke Xu and
• Yuzhe Liu

Beilstein J. Nanotechnol. 2022, 13, 1256–1267, doi:10.3762/bjnano.13.104

• -enhanced Raman spectroscopy and fluorescence microscopy. They can open new avenues for characterizing nano-objects and make it possible to study chemical and physical phenomena occurring at the nanoscale. Following the preparation and application of monometallic nanowire probes, Fang et al. [36] proposed a

Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications

• Aisha Kanwal,
• Naheed Bibi,
• Sajjad Hyder,
• Arif Muhammad,
• Hao Ren,
• Jiangtao Liu and
• Zhongli Lei

Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• = 365 nm, λem = 482 nm for SPION@bPEI). The fluorescence microscopy images were obtained by using an Olympus-excellence RT Life Science microscope (λexc = 488 nm, λem = 510 nm for GFP). Results and Discussion Delivery of therapeutic PIC to cancer cells with SPION@bPEI and in vitro optical imaging Highly

• PIC delivered into the cells, which is excellent. One of the most attractive features of this SPION@bPEI is the intense blue emission, which makes additional fluorescent tagging unnecessary. Fluorescence microscopy images of the cells treated with SPION@bPEI show a strong intracellular optical signal

• triggering of an intrinsic apoptotic pathway [55][56][57]. Most probably, the weak luminescence and high toxicity at the required concentrations to observe the fluorescence signal are the reasons why bPEI luminescence has not been detected by fluorescence microscopy studies before [18][58]. Targeted delivery

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

• Sadaf Mushtaq,
• Khuram Shahzad,
• Tariq Saeed,
• Anwar Ul-Hamid,
• Bilal Haider Abbasi,
• Nafees Ahmad,
• Waqas Khalid,
• Muhammad Atif,
• Zulqurnain Ali and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99

• HT144) were quantitatively determined by fluorescence microscopy using acridine orange and propidium iodide (AOPI) staining. Cells were treated with drug-loaded NPs for 3 h at concentration values of 5 and 10 μg/mL. NTC, NPs-PMA (10 μg/mL) and free drugs (DOX and MTX, 5 μM each) were included as

The role of convolutional neural networks in scanning probe microscopy: a review

• Ido Azuri,
• Irit Rosenhek-Goldian,
• Neta Regev-Rudzki,
• Georg Fantner and
• Sidney R. Cohen

Beilstein J. Nanotechnol. 2021, 12, 878–901, doi:10.3762/bjnano.12.66

• image types was achieved, such as in images of cells and worms, enabling efficient cell-counting and viability prediction, as well as of silver nanowires. Image classification and segmentation CNNs are popular for cell classification. A CNN model based on actin-labeled fluorescence microscopy images

• , fluorescence microscopy, and serial block-face scanning electron microscopy (SBEM) micrographs [108]. Deep learning techniques have been developed for segmentation and tracing of neurons in volume electron microscopy for synaptic connectivity reconstruction. Mitochondria, synapses, axons, dendrites, spines

• labels in transmitted-light images of unlabeled biological samples. ISL can predict labels for nuclei, cell type, and cell state [116]. Image restoration and de-noising: In fluorescence microscopy, imaging speed, spatial resolution, light exposure, and imaging depth are all limited by the optics of the

Fusion of purple membranes triggered by immobilization on carbon nanomembranes

• René Riedel,
• Natalie Frese,
• Fang Yang,
• Martin Wortmann,
• Raphael Dalpke,
• Daniel Rhinow,
• Norbert Hampp and
• Armin Gölzhäuser

Beilstein J. Nanotechnol. 2021, 12, 93–101, doi:10.3762/bjnano.12.8

• CNM and a c-His PM. The c-His PM consists of BR (purple) with a lipid bilayer between the BR molecules. The chemical structure shows the linker used to build a complex between NTA CNM and c-His PM. (b) Fluorescence microscopy of NTA-functionalized NBPT CNMs incubated with a fluorescent protein

Bio-imaging with the helium-ion microscope: A review

• Matthias Schmidt,
• James M. Byrne and
• Ilari J. Maasilta

Beilstein J. Nanotechnol. 2021, 12, 1–23, doi:10.3762/bjnano.12.1

• holds promise to detect fluorescent biomarkers with better resolution than that achievable even with the most advanced super-resolution optical microscopy techniques, for instance, stimulated emission depletion microscopy [51]. In particular, it may allow for correlating fluorescence microscopy with HIM

• that coating, as required for SEM, introduces artefacts such as a granular structure on the cell surfaces and a partial closing of pores, thus highlighting one of the benefits of HIM. Bazou et al. also studied tumour cell-induced platelet aggregation by fluorescence microscopy and HIM. In this study

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• vector in HL-1 cells, we used plasmid-enhanced green fluorescent protein (pEGFP) as a model plasmid and evaluated the transfection efficiency via fluorescence microscopy. First, we used HAp (1 µg/mL) mixed with 0.075, 0.30, and 0.75 µg pEGFP, based on our previous results with endothelial cells

• . Fluorescence microscopy images showed that the highest transfection efficiency was observed with 0.75 µg of pEGFP (Figure 3). No gene expression was obtained by adding only pEGFP in HL-1 cells (data not shown). The results shown in Figure 3b demonstrated that the transfection efficiency of pEGFP increased in a

• commonly used as an indicator of the macropinocytic pathway [25], enabling the quantification of macropinocytosis. The quantification of TMR–dextran was performed by measuring the fluorescence intensity per cell, using fluorescence microscopy images, in accordance with a previous report. As shown in Figure

Silver-decorated gel-shell nanobeads: physicochemical characterization and evaluation of antibacterial properties

• Marta Bartel,
• Katarzyna Markowska,
• Marcin Strawski,
• Krystyna Wolska and
• Maciej Mazur

Beilstein J. Nanotechnol. 2020, 11, 620–630, doi:10.3762/bjnano.11.49

• . The analyses were conducted using the LIVE/DEAD BacLight bacterial viability kit and the samples were imaged with confocal fluorescence microscopy [50]. The test uses the properties of fluorescent dyes, namely, green SYTO 9 and red propidium iodide. The SYTO 9 stain labels the bacteria with intact

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• bioimaging in preclinical, clinical evaluation and patient treatment has encouraged extensive investigation to develop new imaging methods [3][4]. Among several imaging techniques, fluorescence microscopy has evolved as a widely used non-invasive method to visualize real-time biological processes with high

• spatial resolution [5][6]. The image quality of biological structures under fluorescence microscopy also depends on the performance of the fluorophores. Furthermore, bioimaging of cells and tissues faces additional challenges due to background autofluorescence generated from the intrinsic emission of

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• fluorescence was greater (p < 0.05) in cells treated with target-lipo. We next confirmed the flow cytometric results using fluorescence microscopy. Following 5 min exposure to either control (Figure 4b) or target-liposomes (Figure 4c), A549-GRPR cells displayed greater cellular fluorescence signals in the FL

• were acquired in the gated population, and analysed using CytExpert software (v2.3, Beckman Coulter, USA). Fluorescence microscopy Cells were seeded onto 16 mm coverslips, and incubated for 24 h. Cells were washed (×3) with PBS for 5 min at RT and treated with 1 µg/mL (total lipid) of fluorescein

Small protein sequences can induce cellular uptake of complex nanohybrids

• Jan-Philip Merkl,
• Malak Safi,
• Christian Schmidtke,
• Fadi Aldeek,
• Johannes Ostermann,
• Tatiana Domitrovic,
• Sebastian Gärtner,
• John E. Johnson,
• Horst Weller and
• Hedi Mattoussi

Beilstein J. Nanotechnol. 2019, 10, 2477–2482, doi:10.3762/bjnano.10.238

• consisting of 100 nM QD solution, 2 equivalents of LA-ZW-AuNP per QD and 14 equivalents His6-MBP-γ per AuNPs, were incubated with the cell culture for 1 h. Following rinsing the culture was imaged using epifluorescence and confocal fluorescence microscopy. A pronounced intracellular uptake of the hybrids was

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

• Saban Kalay,
• Yurij Stetsyshyn,
• Volodymyr Donchak,
• Khrystyna Harhay,
• Ostap Lishchynskyi,
• Halyna Ohar,
• Yuriy Panchenko,
• Stanislav Voronov and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

• )-functionalized BNNTs Fluorescence microscopy of living cells has become an integral part of modern cell biology. Most often cellular imaging is provided using fluorescent labels, including fluorescent dyes, nanoparticles, nanocomposites or proteins [55]. This label must meet certain criteria, such as

• uptake of P(AA-co-FA)-functionalized BNNTs into human normal prostate epithelium (PNT1A) and human prostate cancer (DU145) cell lines, we used fluorescence microscopy with excitation at 490 nm and emission at 520 nm (Figure 9). The autofluorescence of healthy cells and cancer cells was strictly avoided

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• distribution of molecules. However, many native tissues are not homogeneously stiff and it is not clear whether the controlled presentation of rigid and flexible material axes on the substrate governs the cytoskeletal and nuclear morphology [14]. Several techniques such as fluorescence microscopy [14][15

• substrate. Fluorescence microscopy was used to investigate the role of the substrate stiffness in controlling the cell properties, including the size of the cell areas and the alignment of adherent cells (Figure 3a–l). We examined the morphology of the L929 cells by quantifying the cell spreading areas and

• deformation with increasing EBL exposure dose. Fluorescence microscopy images show that the L929 cells cultured on the patterned stiffness surfaces have a modified shape. However, the cell shape does not change as a function of the different elasticities. Furthermore, the L929 cells align along the stripes of

Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

• Sabrina Barbosa de Souza Ferreira,
• Gustavo Braga,
• Évelin Lemos Oliveira,
• Jéssica Bassi da Silva,
• Hélen Cássia Rosseto,
• Lidiane Vizioli de Castro Hoshino,
• Mauro Luciano Baesso,
• Wilker Caetano,
• Craig Murdoch,
• Helen Elizabeth Colley and
• Marcos Luciano Bruschi

Beilstein J. Nanotechnol. 2019, 10, 2304–2328, doi:10.3762/bjnano.10.222

• . Consequently, it was evaluated only for the CUR systems. The retention of the systems without CUR have already been evaluated by a similar method, where the formulations were marked with FITC-dextran and the retention was investigated by fluorescence microscopy [70]. The cumulative formulation percentage

BergaCare SmartLipids: commercial lipophilic active concentrates for improved performance of dermal products

• Florence Olechowski,
• Rainer H. Müller and
• Sung Min Pyo

Beilstein J. Nanotechnol. 2019, 10, 2152–2162, doi:10.3762/bjnano.10.208

• suspension was prepared containing 0.2% curcumin. Curcumin has many positive effects on the skin [31][32] and is at the same time fluorescent, allowing for a good and easy detection in the skin by fluorescence microscopy. The suspension was applied to pig ear skin in a covered Franz cell, incubated for 24 h

• and then skin slices were investigated by normal light and fluorescence microscopy (Figure 8, left column). Figure 8 (upper row) shows the fluorescence microscopy images. The lower row shows overlays of fluorescence and light microscopy images, allowing to locate the fluorescence in the epidermis. For

• . Penetration of curcumin into pig ear skin, vertical pig ear slices, fluorescence microscopy images (upper row) and overlay of fluorescence and light microscopy images (lower row); after application of 0.2% curcumin suspension in SmartLipids (left column), 2.0% curcumin micrometer-size crystal suspension

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

• illumination [7]. The characterization of single NV centers became popular at the end of the 1990s. It was demonstrated that the fluorescence of single NV centers can be detected by room-temperature fluorescence microscopy and that the defect shows perfect photostability [8]. Room-temperature optically

• allows real-time imaging of bacteria magnetosome chains with a direct correlation to the optical image. Single magnetosomes were thus resolved by the technique on a 100 μm wide field [52]. An alternative approach to fluorescence microscopy is magnetic imaging of cells, labeled or targeted, using MNPs

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• −53.8 ± 2.4 mV upon antibody coating of the particles. The zeta potential value of VCAM1-PEG5000Au-CPMV becomes more negative [38], which is consistent with the literature [42]. In vitro fluorescent cell imaging Confocal fluorescence microscopy was performed on the cell lines to demonstrate the

• . Fluorescence microscopy confirmed that the VCAM1-PEG5000Au-CPMV can selectively bind to their target, whereas, the IgG-PEG5000Au-CPMV control did not show any fluorescence signal, which is indicative that no binding to the surface of the cells occurred (Figure 4A). The merged confocal microscopy image in

• holder. (C) UV–vis spectrum of the SPR bands of Au-CPMV; λmax = 535 nm for particles with a diameter of 50 nm (purple line), λmax = 552 nm for particles with a diameter of 70 nm (red line) and λmax = 572 nm for particles with a diameter of 100 nm (green line). Confocal fluorescence microscopy images of